HSF1 Antibody

Affinity purified Anti-HSF1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the n-terminal portion of human HSF1 protein.

Anti-HSF1 is directed against human HSF1 at a n-terminus position. This product is an affinity purified antibody produced by immunoaffinity chromatography using peptide coupled to agarose beads. A BLAST analysis was used to suggest reactivity with this protein in mouse and bovine species based on 100% homology for the immunogen sequence.

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Anti-Heat Shock Factor 1 Antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band of 57 kDa in size corresponding to HSF1 by western blotting in the appropriate cell lysate or extract. Tested using positive control A431 Whole Cell Lysate p/n W09-000-361.