HSF1 Antibody
HSF1 antibody
Product Specifications
Product Name Alternative
Rabbit anti-HSF1 antibody, HSF-1, HSF 1, heat shock factor protein 1, HSTF1, HSTF 1, HSTF-1, heat shock transcription factor 1
UniProt
Q00613
Reactivity
Human
Immunogen
Affinity purified Anti-HSF1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the n-terminal portion of human HSF1 protein.
Target
HSF1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Epigenetics & Chromatin
Concentration
0.96 mg/ml
Dilution
ELISA: 5 ug/ml, WB: 1 ug/ml
Purity
Anti-HSF1 is directed against human HSF1 at a n-terminus position. This product is an affinity purified antibody produced by immunoaffinity chromatography using peptide coupled to agarose beads. A BLAST analysis was used to suggest reactivity with this protein in mouse and bovine species based on 100% homology for the immunogen sequence.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-Heat Shock Factor 1 Antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band of 57 kDa in size corresponding to HSF1 by western blotting in the appropriate cell lysate or extract. Tested using positive control A431 Whole Cell Lysate p/n W09-000-361.
Tested Applications
ELISA, WB
NCBI Accession Number
NP_005517.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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