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EZH1 Antibody

Ezh1 antibody

Product Specifications

Product Name Alternative

Rabbit anti-EZH1 antibody, KIAA0388, Histone-lysine N-methyltransferase EZH1, ENX-2, Enhancer of zeste homolog 1, EZH-1, EZH 1

UniProt

Q92800

Reactivity

Human, Mouse

Immunogen

Affinity purified Anti-EZH1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the internal portion of human EZH1 protein.

Target

EZH1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Epigenetics & Chromatin

Concentration

0.95 mg/ml

Dilution

ELISA: 5.0 ug/ml, WB: 1.0 ug/ml

Purity

Anti-EZH1 is directed against human EZH1 at an internal position. This product is an affinity purified antibody produced by immunoaffinity chromatography using peptide coupled to agarose beads. A BLAST analysis was used to suggest reactivity with this protein in mouse, bovine, and orangutan species based on 100% homology for the immunogen sequence.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Affinity purified Anti-EZH1 antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~86 kDa in size corresponding to Ezh1 by western blotting in the appropriate cell lysate or extract. Testing using positive control NIH/3T3 nuclear extract p/n W10-001-A74.

Tested Applications

ELISA, WB

NCBI Accession Number

NP_001982.2

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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