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Nfe2l2 Antibody

Nrf2 antibody

Product Specifications

Product Name Alternative

Rabbit anti-Nrf2 antibody, Nrf 2, Nrf-2 Antibody, Nuclear factor erythroid 2-related factor 2, NF-E2-related factor 2, NFE2-related factor 2, Nuclear factor, erythroid derived 2, Nfe2l2, HEBP1

UniProt

Q60795

Reactivity

Human, Mouse

Immunogen

Anti-NRF-2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding near the c-term region.

Target

Nfe2l2

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Concentration

1.0 mg/ml

Dilution

ELISA: 5 ug/ml, WB: 1.0 ug/ml

Purity

This affinity-purified antibody is directed against mouse Nrf2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with Nrf2 proteins from human, mouse, rat and bovine based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This affinity purified Nrf2 antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 66.9 kDa in size corresponding to Nrf-2 proteins by western blotting in the appropriate cell lysate or extract.

Tested Applications

ELISA, WB

NCBI Accession Number

NP_035032.1

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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