Pax7 Antibody
Pax7 antibody
Product Specifications
Product Name Alternative
Rabbit anti-Pax7 antibody, Paired box protein Pax-7, Pax 7, Pax-7
UniProt
P47239
Reactivity
Human, Mouse
Immunogen
Pax7 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Pax7 protein.
Target
Pax7
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Stem Cell & Developmental Biology
Concentration
1.0 mg/ml
Dilution
ELISA: 1:10,000 - 1:40:000, WB: 1.0 ug/ml
Purity
Anti-Pax-7 was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross reactivity with Pax7 protein from mouse (100% homology) and human (66.7% homology) for the immunizing sequence. Cross-reactivity with Pax7 from other sources has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-Pax7 antibody has been tested in ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 54.9 kDa in size corresponding to Pax7 by western blotting in the appropriate cell lysate or extract.
Tested Applications
ELISA, WB
NCBI Accession Number
NP_035169.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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