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Slc2a2 Antibody

GLUT2 antibody

Product Specifications

Product Name Alternative

Rabbit anti-Glut2 antibody, Solute carrier family 2 facilitated glucose transporter member 2, Glucose transporter type 2 liver, GLUT-2 antibody, Slc2a2, Glut 2 Antibody

UniProt

P14246

Reactivity

Human, Mouse

Immunogen

This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal domain of mouse Glut2 protein.

Target

Slc2a2

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

1.0 mg/mL

Dilution

ELISA: 1:30,000-1:90:000, IHC: 4μg/mL, WB: 1:500-1:1000

Purity

This affinity-purified Glut-2 antibody is directed against Glut2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with Glut2 from mouse and rat based on a 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Anti-Glut-2 antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 57.1 kDa in size corresponding to Glut2 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.

Tested Applications

ELISA, IF, IHC, WB

NCBI Accession Number

NP_112474.2

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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