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MAPK3 Antibody

P44 Map Kinase antibody

Product Specifications

Product Name Alternative

ERK1 antibody, ERT 2 antibody, ERT2 antibody, Extracellular Signal Regulated Kinase 1 antibody, Extracellular signal related kinase 1 antibody, HGNC6877 antibody, HS44KDAP antibody, HUMKER1A antibody

UniProt

P27361

Reactivity

Mouse

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the carboxy terminal end of human p44 MAP Kinase (ERK1) protein.

Target

MAPK3

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cell Biology

Concentration

1.0 mg/mL

Dilution

ELISA: 1:2,000 to 1:10,000, IP: 5.0 μg/mg sample, WB: 1:1,000-1:,2000

Purity

This affinity-purified antibody is directed against the human p44 MAP Kinase (ERK1) protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with p44 MAP Kinase (ERK1) proteins from human, bovine, rabbit, rat and mouse. No reactivity is observed against p42 MAP Kinase (ERK2) . Reactivity against homologues from other sources is not known.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This affinity purified antibody has been tested for use in ELISA, immunoprecipitation and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band approximately 44 kDa in size corresponding to p44 MAP Kinase (ERK1) by western blotting in the appropriate cell lysate or extract.

Tested Applications

ELISA, IP, WB

NCBI Accession Number

91718899

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0

Isotype

IgG

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