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RPS6KA1 Antibody

P90 RSK1 antibody

Product Specifications

Product Name Alternative

90 kDa ribosomal protein S6 kinase 1 antibody, dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody, dJ590P13.1 antibody, EC 2.7.11.1 antibody, HU 1 antibody, RSK1

UniProt

Q15418

Reactivity

Mouse

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-terminal region of human RSK1 protein.

Target

RPS6KA1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Signal Transduction

Concentration

1.0 mg/mL

Dilution

ELISA: 1:2,000 - 1:5,000, WB: 1:200 - 1:1000

Purity

This affinity-purified antibody is directed against the human RSK1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with RSK1 proteins from human, rat and mouse. Partial reactivity may occur against RSK2 and RSK3 but has not been tested. Reactivity against homologues from other sources is not known.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This affinity purified antibody has been tested for use in ELISA, western blot, and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band approximately 88 kDa in size corresponding to RSK1 by western blotting in the appropriate cell lysate or extract.

Tested Applications

ELISA, WB

NCBI Accession Number

NP_001006666.1

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 50% (v/v) Glycerol; Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0

Isotype

IgG

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