Lysine Methylated Antibody
Lysine Methylated antibody
Product Specifications
Product Name Alternative
Dimethylysine antibody, Methyl lysine antibody, N epsilon dimethyl lysine antibody, N epsilon mono methyllysine antibody, N epsilon trimethyl lysine antibody, N (epsilon) trimethyl lysine antibody, Trimethyl lysine antibody
Immunogen
This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with methylated lysine conjugated KLH.
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Pharmacology & Drug Discovery
Concentration
250 μg/ml
Dilution
ELISA: 1:1,000 - 1:20,000, WB: 1:500 - 1:3,000
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using methylated lysine peptide coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG. Reactivity is specific for methylated lysine residues (mono, di) . The cross reactivity with tri-methyl lysine has not been tested. There are no cross reactions with acetylated proteins.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This affinity-purified antibody is suitable for use in ELISA, immunohistochemistry, western blotting and immunoprecipitation assays. Although not tested, this antibody is likely functional in RIA, and flow cytometry.
Tested Applications
ELISA, WB
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 50% (v/v) Glycerol; Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Isotype
IgG
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