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Alpha-1-Trypsin Antibody

Alpha-1-Trypsin antibody

Product Specifications

Product Name Alternative

Rabbit anti-alpha-1-antitrypsin antibody, A1A antibody, A1AT, AAT, Alpha 1 antiproteinase antibody, Alpha 1 antitrypsin, Alpha 1 protease inhibitor, alpha-1-AT antibody, alpha1 PI antibody, alpha1 proteinase inhibitor antibody, Serpin A1, Anti-α-1-antitrypsin

UniProt

P01009

Reactivity

Human

Immunogen

Human Alpha-1-anti-Trypsin

Target

SERPINA1

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

1.0mg/mL

Dilution

ELISA: 1:380,000-1:460,000, IHC: 1:500 - 1:2,500, WB: 1:2,000 - 1:10,000

Purity

Anti-Human ALPHA-1-ANTI-TRYPSIN Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigen resins attached to a solid phase. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Analysis by SDS-PAGE was used to determine that this preparation is substantially free of aggregates and shows a banding pattern consistent with purified Rabbit IgG.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Human ALPHA-1-ANTI-TRYPSIN Antibody has been tested by LEISA, SDS-PAGE, and western blot. This product is assayed against 1.0 μg of Human Alpha-1-anti-Trypsin in a standard ELISA using Peroxidase conjugated Affinity Purified Donkey anti-Rabbit IgG and TMB as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:50,000 is suggested for this product.

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

NP_000286.3

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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