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IKBKG Antibody

NEMO antibody

Product Specifications

Product Name Alternative

Rabbit anti-NEMO antibody, IKBKG Antibody, NF-kappa-B essential modulator, FIP3, NEMO, regulatory subunit of the IKK core complex, I-kappa-B kinase subunit gamma, IKK-gamma, IKKG, IkB kinase-associated protein 1, IKKAP1, rabbit anti-IKK gamma

UniProt

Q9Y6K9

Reactivity

Human

Immunogen

Anti-NEMO was affinity purified from whole rabbit serum prepared by repeated immunizations with a recombinant protein of human NEMO.

Target

IKBKG

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

1.1 mg/ml

Dilution

ELISA: 1:50,000 - 1:400,000, IP: 5μg, WB: 1.0 ug/ml

Purity

Anti-NEMO is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody detects human NEMO. Cross reactivity with NEMO from other sources is unknown.

Form

Liquid (sterile filtered)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Anti-NEMO antibody has been tested by western blot and is suitable for immunoprecipitation and ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48kDa in size corresponding to endogenous NEMO protein by western blotting in the appropriate cell lysate or extract.

Tested Applications

ELISA, IP, WB

NCBI Accession Number

NP_003630.1

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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