IKBKG Antibody
NEMO antibody
Product Specifications
Product Name Alternative
Rabbit anti-NEMO antibody, IKBKG Antibody, NF-kappa-B essential modulator, FIP3, NEMO, regulatory subunit of the IKK core complex, I-kappa-B kinase subunit gamma, IKK-gamma, IKKG, IkB kinase-associated protein 1, IKKAP1, rabbit anti-IKK gamma
UniProt
Q9Y6K9
Reactivity
Human
Immunogen
Anti-NEMO was affinity purified from whole rabbit serum prepared by repeated immunizations with a recombinant protein of human NEMO.
Target
IKBKG
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1.1 mg/ml
Dilution
ELISA: 1:50,000-1:400,000, IP: 5μg, WB: 1:500-1:1000
Purity
Anti-NEMO is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody detects human NEMO. Cross reactivity with NEMO from other sources is unknown.
Form
Liquid (sterile filtered)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Anti-NEMO antibody has been tested by western blot and is suitable for immunoprecipitation and ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48kDa in size corresponding to endogenous NEMO protein by western blotting in the appropriate cell lysate or extract.
Tested Applications
ELISA, IP, WB
NCBI Accession Number
NP_003630.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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