VHH Antibody

VhH antibody

Product Specifications

Product Name Alternative

Rabbit anti-VHH Antibody, single-domain antibody (sdAb), camelid VHH antibody

Reactivity

Camelus

Immunogen

This antibody was prepared from whole rabbit serum produced by repeated immunizations with a VHH camelid domain protein.

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

1.3 mg/mL

Dilution

ELISA: 1:10,000-1:50,000, WB: 1:1,000-1:5,000

Purity

Anti-VHH is directed against the llama VHH protein fragment. The product was purified from monospecific antiserum by protein A chromatography. Anti-VHH antibody detects recombinant VHH proteins, native Llama IgG2 and native Llama IgG3.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Anti-VhH antibody has been tested by ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Some cross-reactivity to E.coli proteins may be observed.