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MAPK1 Antibody (Biotin)

ERK1/2 antibody (Biotin)

Product Specifications

Product Name Alternative

Mouse anti-ERK2 antibody biotin conjugation, biotin conjugated mouse anti-ERK2 antibody, MAPK1, ERK, ERK-2, ERK2, MAPK2, P42MAPK, PRKM1, PRKM2, Biotin, ERK-2 Antibody

UniProt

P28482

Reactivity

Human, Mouse, Rat

Immunogen

Anti-ERK2 Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near an internal region of ERK2 conjugated to KLH.

Target

MAPK1

Clonality

Monoclonal

Clone

3H9.A1.A8

Conjugation

Biotin

Field of Research

Cell Biology

Concentration

1 mg/mL

Dilution

ELISA: 1:40,000, WB: 1μg/mL

Purity

This Biotin conjugated protein A purified mouse monoclonal antibody reacts specifically with human ERK2. Anti-ERK2 is purified from tissue culture supernatant by protein A purification. Cross reactivity is expected to occur with human, mouse, and rat based on sequence identity of the peptide immunogen. This antibody does not react with the ERK1 isoform.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Anti-ERK 2 (MOUSE) biotin conjugated antibody is suitable for use in ELISA and Western Blotting. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 41 kDa.

Tested Applications

ELISA, WB

Host or Source

Mouse

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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