MAP2K1/MAP2K2 Antibody
MEK1 (phospho-S222) / MEK2 | (phospho-S226) antibody
Product Specifications
Product Name Alternative
Mouse anti-MEK1 pS222/MEK2 pS226 Antibody, MAP2K, MEK, MEK1, MKK1, PRKMK1, MEK-1 Antibody, MAP2K2, MEK2, MKK2, PRKMK2, CFC4, MEK-2 Antibody, MEK1 and MEK2 antibody, phosphor MEK1/2
UniProt
Q02750
Reactivity
Human, Mouse, Rat
Immunogen
Anti-MEK1 pS222/MEK2 pS226 Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues surrounding the S222-226 phosphorylation site conjugated to KLH.
Target
MAP2K1/MAP2K2
Clonality
Monoclonal
Clone
17C9.E1.H2.D7.H7.D5
Conjugation
Unconjugated
Field of Research
Signal Transduction
Concentration
1.0 mg/mL
Dilution
ELISA: 1:40,000
Purity
This protein A purified mouse monoclonal antibody specifically binds to the human MEK1 pS222 and MEK2 pS226 phosphorylated sites. Anti-MEK1 pS222/MEK2 pS226 is purified from tissue culture supernatant by protein A purification. Cross reactivity is expected to occur with human, mouse, and rat based on sequence identity of the peptide immunogen.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-MEK1 pS222/MEK2 pS226 (MOUSE) antibody has been tested in ELISA and Western Blot. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 43.5 kDa.
Tested Applications
ELISA, WB
Host or Source
Mouse
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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