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MAP2K1/MAP2K2 Antibody

MEK1 (phospho-S222) / MEK2 | (phospho-S226) antibody

Product Specifications

Product Name Alternative

Mouse anti-MEK1 pS222/MEK2 pS226 Antibody, MAP2K, MEK, MEK1, MKK1, PRKMK1, MEK-1 Antibody, MAP2K2, MEK2, MKK2, PRKMK2, CFC4, MEK-2 Antibody, MEK1 and MEK2 antibody, phosphor MEK1/2

UniProt

Q02750

Reactivity

Human, Mouse, Rat

Immunogen

Anti-MEK1 pS222/MEK2 pS226 Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues surrounding the S222-226 phosphorylation site conjugated to KLH.

Target

MAP2K1/MAP2K2

Clonality

Monoclonal

Clone

17C9.E1.H2.D7.H7.D5

Conjugation

Unconjugated

Field of Research

Signal Transduction

Concentration

1.0 mg/mL

Dilution

ELISA: 1:40,000

Purity

This protein A purified mouse monoclonal antibody specifically binds to the human MEK1 pS222 and MEK2 pS226 phosphorylated sites. Anti-MEK1 pS222/MEK2 pS226 is purified from tissue culture supernatant by protein A purification. Cross reactivity is expected to occur with human, mouse, and rat based on sequence identity of the peptide immunogen.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Anti-MEK1 pS222/MEK2 pS226 (MOUSE) antibody has been tested in ELISA and Western Blot. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 43.5 kDa.

Tested Applications

ELISA, WB

Host or Source

Mouse

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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