HBD Antibody
Hemoglobin beta A-2 antibody
Product Specifications
Product Name Alternative
Mouse anti-HbA-2 antibody, mouse anti-hemoglobin antibody, Hemoglobin Subunit Delta, Hemoglobin Delta Chain 4, Delta-Globin 4, HbD, HbA-2 Antibody, Sickle Cell Disease (SCD)
UniProt
P02042
Reactivity
Human
Immunogen
Anti-Hemoglobin beta A-2 Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-terminus of Hb δ-subunit conjugated to KLH.
Target
HBD
Clonality
Monoclonal
Clone
21G1.F1.B9.G9.D11
Conjugation
Unconjugated
Concentration
1.00 mg/ml
Dilution
ELISA: 1:20,000, WB: 1ug/mL
Purity
This protein A purified mouse monoclonal antibody reacts specifically with human HbA-2 delta isoform. Anti-HbA-2 is purified from tissue culture supernatant by protein A purification. Blast analysis shows 100% homology to Human, Pan troglodytes, Pan paniscus, Gorilla gorilla gorilla, and Hylobates lar. This antibody does not react with the HbA, HbS, HbC, or HbF isoforms.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-Hemoglobin beta A-2 (MOUSE) antibody has been tested by ELISA, SDS-Page, and Western Blotting. This antibody is designed for use in lateral flow. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 16 kDa.
Tested Applications
ELISA, WB
NCBI Accession Number
NP_000510.1
Host or Source
Mouse
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
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