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HBD Antibody

Hemoglobin beta A-2 antibody

Product Specifications

Product Name Alternative

Mouse anti-HbA-2 antibody, mouse anti-hemoglobin antibody, Hemoglobin Subunit Delta, Hemoglobin Delta Chain 4, Delta-Globin 4, HbD, HbA-2 Antibody, Sickle Cell Disease (SCD)

UniProt

P02042

Reactivity

Human

Immunogen

Anti-Hemoglobin beta A-2 Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-terminus of Hb δ-subunit conjugated to KLH.

Target

HBD

Clonality

Monoclonal

Clone

21G1.F1.B9.G9.D11

Conjugation

Unconjugated

Concentration

1.00 mg/ml

Dilution

ELISA: 1:20,000, WB: 1ug/mL

Purity

This protein A purified mouse monoclonal antibody reacts specifically with human HbA-2 delta isoform. Anti-HbA-2 is purified from tissue culture supernatant by protein A purification. Blast analysis shows 100% homology to Human, Pan troglodytes, Pan paniscus, Gorilla gorilla gorilla, and Hylobates lar. This antibody does not react with the HbA, HbS, HbC, or HbF isoforms.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Anti-Hemoglobin beta A-2 (MOUSE) antibody has been tested by ELISA, SDS-Page, and Western Blotting. This antibody is designed for use in lateral flow. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 16 kDa.

Tested Applications

ELISA, WB

NCBI Accession Number

NP_000510.1

Host or Source

Mouse

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

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