MAP2K1 Antibody
MEK1 antibody
Product Specifications
Product Name Alternative
Mouse anti-MEK1 antibody, MAP2K, MEK, MEK 1, MKK1, PRKMK1, MEK-1 Antibody
UniProt
Q02750
Reactivity
Human, Mouse, Rat
Immunogen
Anti-MEK1 Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the C-terminus conjugated to KLH.
Target
MAP2K1
Clonality
Monoclonal
Clone
13B6.G12
Conjugation
Unconjugated
Field of Research
Signal Transduction
Concentration
1.0 mg/ml
Dilution
ELISA: 1:40,000, WB: 1μg/mL
Purity
This protein A purified mouse monoclonal antibody reacts specifically with human MEK1. Anti-MEK1 is purified from tissue culture supernatant by protein A purification. Cross reactivity is expected to occur with human, mouse, and rat based on sequence identity of the peptide immunogen. This antibody does not react with the MEK2 isoform.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-MEK 1 (MOUSE) antibody is tested in ELISA and Western Blotting. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 43.5 kDa.
Tested Applications
ELISA, WB
Host or Source
Mouse
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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