ACTB Antibody
Beta Actin antibody
Product Specifications
Product Name Alternative
Mouse anti-beta Actin Antibody, Actin Antibody, Loading Control Antibody, beta actin, ß actin, anti-beta actin antibody
UniProt
P60709
Reactivity
Human, Mouse, Rat
Immunogen
Anti-beta Actin (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with ß-Actin peptide near the N-Terminus followed by hybridoma development.
Target
ACTB
Clonality
Monoclonal
Clone
3H2.E4.E12
Conjugation
Unconjugated
Field of Research
Cancer Biology
Concentration
1.0 mg/mL
Dilution
ELISA: 1:5000-1:50000, WB: 1 μg/mL
Purity
Anti-beta Actin was purified from mouse ascites by Protein A chromatography followed by extensive dialysis against the buffer stated above. beta Actin antibody is specific for human and mouse ß Actin protein. Expected to cross-react with a wide range of species due to sequence homology (human, mouse, rat, swine) . A BLAST analysis was used to suggest that this antibody would react with beta Actin from a wide range of organisms, including most vertebrates. Cross-reactivity with other sources has not been determined.
Form
Liquid (sterile filtered)
References & Citations
Yisheng Chen 1, Zhongcheng Fan 2, Zhiwen Luo 1, Xueran Kang 3, Renwen Wan 1, Fangqi Li 1, Weiwei Lin 4, Zhihua Han 5, Beijie Qi 1, Jinrong Lin 1, Yaying Sun 1, Jiebin Huang 6, Yuzhen Xu 7, Shiyi Chen Impacts of Nutlin-3a and exercise on murine double minute 2-enriched glioma treatment Neural Regen Res, (2024)
Pubmed id
38989952
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Anti-beta Actin antibody has been tested for use in ELISA, immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user.
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_001092.1
Host or Source
Mouse
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG2a
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