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Mouse IgG2b Antibody Texas Red Conjugated Pre-adsorbed

Mouse IgG2b antibody (Texas Red)

Product Specifications

Product Name Alternative

Goat anti-MOUSE IgG2b Antibody Texas Red Conjugation, goat anti-Mouse IgG2b (gamma 2b) Texas Red Conjugated Antibody

Reactivity

Mouse

Immunogen

Mouse IgG2b heavy chain

Clonality

Polyclonal

Conjugation

Texas Red

Concentration

1.0 mg/mL

Dilution

FLISA: 1:10,000 - 1:50,000, IHC: 1:1,000 - 1:5,000, IF: 1:1,000 - 1:5,000, WB: 1:4,000 - 1:20,000

Purity

Anti-MOUSE IgG2b (Gamma 2b chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG2b. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Mouse IgG2b Texas Red Conjugated antibody has been tested by dot blot and is suitable for ELISA, Immunohistochemistry, western blotting, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting as well as other anti IgG2a antibody based assays. Mouse IgG2b secondary antibody is available in a variety of formats. This product is suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Tested Applications

DOT, FLISA, IF, IHC, WB

Host or Source

Goat

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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