POLB Antibody
POLB antibody
Product Specifications
Product Name Alternative
Rabbit anti-POLB antibody, rabbit anti-DNA polymerase beta antibody, DNA polymerase ß, POLß, POL-B, POL-ß, pol-beta, beta-pol
UniProt
P06746
Reactivity
Human
Immunogen
This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of the POLß protein.
Target
POLB
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Epigenetics & Chromatin
Concentration
1.0 mg/mL
Dilution
ELISA: 1:50,000, WB: 0.5 μg/mL
Purity
This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with overexpressed and endogenous POLß protein. A BLAST analysis was used to suggest reactivity with POLß from human, bovine, and Xenopus laevis based on a 100% homology with the immunizing sequence. Cross-reactivity with POLß from other sources has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This affinity purified POLß antibody has been tested by ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 38.1 kDa in size corresponding to POLß by western blotting in the appropriate cell lysate or extract.
Tested Applications
ELISA, WB
NCBI Accession Number
NP_002681.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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