Llama IgG1 isotype control Biotin Antibody

Llama IgG1 isotype control has been prepared from llama serum by multiple chromatography steps using a combination of protein A and protein G chromatography. Coomassie stained SDS-PAGE of non-reduced unconjugated llama IgG1 shows a band of ~150 kDa whereas the reduced form exhibits ~55 kDa (heavy chain) and ~25 kDa (light chain) . No bands corresponding to unconjugated llama IgG2 or IgG3 are observed. Biotin Conjugated Llama IgG1 was proven by Dot Blot.

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Llama IgG1 Biotin Conjugated isotype control can be utilized as a control or standard reagent in Flow Cytometry, ELISA, and western blotting experiments where determination of sample isotype is important.

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2