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Donkey IgG (H&L) Antibody Alkaline Phosphatase Conjugated

Donkey IgG (H&L) antibody (Alkaline Phosphatase)

Product Specifications

Product Name Alternative

Goat anti-Donkey IgG Antibody alkaline phosphatase conjugation, goat anti-Donkey IgG alkaline phosphatase conjugated Antibody

Reactivity

Donkey

Immunogen

Donkey IgG whole molecule

Clonality

Polyclonal

Conjugation

AP

Field of Research

Signal Transduction

Concentration

1.0 mg/mL

Dilution

ELISA: 1:10,000 - 1:40,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Donkey IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Donkey IgG, and Donkey Serum.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Notes

For research use only.

Applications Notes

Anti-Donkey IgG Alkaline Phosphatase Antibody has been tested by ELISA and dot blot and is suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.

Tested Applications

DOT, ELISA, IHC, WB

Host or Source

Goat

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0

Isotype

IgG

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