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AKT1 Antibody

AKT antibody

Product Specifications

Product Name Alternative

Sheep anti-AKT Antibody, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1

UniProt

P31749

Reactivity

Human

Immunogen

Anti-AKT antibody was affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of human AKT1 protein. A residue of cysteine was added to facilitate coupling.

Target

AKT1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Signal Transduction

Concentration

0.9 mg/mL

Dilution

ELISA: 1:5,000 - 1:20,000, WB: 1:500 - 1:2,000

Purity

This antibody is directed against AKT and reacts with the AKT from human tissues. Based on sequence we expect this antibody to react as well with rat, mouse and chicken AKT.

Form

Liquid (sterile filtered)

Storage Conditions

Store Anti-AKT at -20° C prior to opening. Aliquot antibody and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This Anti-AKT affinity purified antibody is tested for ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user for IF and IHC. Expect a band approximately 55-60 kDa in size corresponding to AKT1 by western blotting in the appropriate cell lysate or extract. Cell Signaling, Neuroscience, Signal Transduction research.

Tested Applications

ELISA, WB

NCBI Accession Number

62241011

Host or Source

Sheep

Preservative

Preservative: 0.1% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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