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Gata1 Antibody

Gata1 antibody

Product Specifications

Product Name Alternative

Rabbit anti-Gata1 antibody, Gata-1, gata 1, Erythroid transcription factor, Eryf1, GATA-binding factor 1, GF-1, NF-E1 DNA-binding protein, Gf-1

UniProt

P17679

Reactivity

Mouse

Immunogen

Anti-Gata1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to mouse Gata1 peptide.

Target

Gata1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Concentration

0.73 mg/mL

Dilution

ELISA: 1:15,000 - 1:75,000, WB: 1:500 - 1:2,000

Purity

Anti-Gata1 is directed against mouse Gata1. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from mouse and rat based on 100% homology for the immunogen sequence and 93% to human. Cross reactivity with Gata1 homologues from other sources has not been determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Gata1 Antibody has been tested by ELISA and Western Blot. Expect a band approximately 43 kDa in size corresponding to mouse Gata1 by western blotting in the appropriate cell lysate or extract. Researchers should determine optimal titers for applications that are not stated below.

Tested Applications

ELISA, WB

NCBI Accession Number

NP_032115.1

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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