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ATG13 Antibody

ATG13 antibody

Product Specifications

Product Name Alternative

Rabbit anti-ATG13 Antibody, ATG-13, ATG 13, Autophagy-related protein 13, KIAA0652

UniProt

O75143

Reactivity

Human

Immunogen

This affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to the S318 region of ATG13.

Target

ATG13

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cell Biology

Concentration

1.24 mg/mL

Dilution

ELISA: 1:220,000-1:250,000, WB: 1:1000

Purity

This affinity-purified antibody is directed against human ATG13 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with ATG13 from human based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 56.6 kDa in size corresponding to human ATG13 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.

Tested Applications

ELISA, WB

NCBI Accession Number

NP_001136145.1

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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