Pogz Antibody

Rabbit anti-POGZ antibody, pogo transposable element with ZNF domain antibody, Suppressor of hairy wing homolog 5, Zinc finger protein 280E, Zinc finger protein 635, SUHW5, ZNF280E, ZNF635

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminus of mouse Pogz protein.

This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is known to react with mouse Pogz protein. A BLAST analysis was used to suggest cross-reactivity with Pogz from human, dog, short-tailed opossum, cattle, rat, chimpanzee, macaque, olive baboon, and chicken sources based on 100% homology with the immunizing sequence. Reactivity with Pogz from other sources has not been determined.

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 155 kDa in size corresponding to Pogz by western blotting in the appropriate cell lysate or extract.