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Fap Antibody

Fibroblast Activation Protein antibody

Product Specifications

Product Name Alternative

Rabbit anti-Fap antibody, Fibroblast activation protein alpha, Prolyl endopeptidase FAP, Dipeptidyl peptidase FAP, Integral membrane serine protease antibody, FAPA antibody, Gelatine degradation protease FAP, Surface-expressed protease, SIMP, Seprase, APCE

UniProt

P97321

Reactivity

Mouse

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse FAP protein.

Target

Fap

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cell Biology

Concentration

1.1 mg/mL

Dilution

ELISA: 1:10,000 - 1:20,000, WB: 1:500 to 1:2,000

Purity

This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is known to react with mouse FAP protein. A BLAST analysis was used to suggest partial cross-reactivity with FAP from human and rat sources based on ~88% homology with the immunizing sequence. Reactivity with FAP from other sources has not been determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 88 kDa in size corresponding to FAP by western blotting in the appropriate cell lysate or extract.

Tested Applications

ELISA, WB

NCBI Accession Number

6679749

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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