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Stat5a Antibody

STAT5 antibody

Product Specifications

Product Name Alternative

Rabbit anti-STAT5 antibody, STAT-5, STAT 5, STAT5A, Signal Transducer and Activator of Transcription 5A antibody, STAT-5A, Transcription factor STAT5A antibody

UniProt

P42230

Reactivity

Human

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues near the carboxy terminus of mouse Stat5a protein.

Target

Stat5a

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

1.2 mg/mL

Dilution

ELISA: 1:25,000, WB: 1:500 - 1:2,000

Purity

This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using peptide coupled to agarose beads. This antibody is specific for mouse Stat5 protein. A BLAST analysis was used to suggest cross-reactivity with Stat5 from human, mouse and rat based on 100% homology with the immunizing sequence. Cross-reactivity with Stat5 from other sources has not been determined.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 91 kDa in size corresponding to phosphorylated Stat5 protein by western blotting in the appropriate cell lysate or extract.

Tested Applications

ELISA, WB

NCBI Accession Number

6755672

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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