Stat5a Antibody
STAT5 antibody
Product Specifications
Product Name Alternative
Rabbit anti-STAT5 antibody, STAT-5, STAT 5, STAT5A, Signal Transducer and Activator of Transcription 5A antibody, STAT-5A, Transcription factor STAT5A antibody
UniProt
P42230
Reactivity
Human
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues near the carboxy terminus of mouse Stat5a protein.
Target
Stat5a
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1.2 mg/mL
Dilution
ELISA: 1:25,000, WB: 1:500 - 1:2,000
Purity
This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using peptide coupled to agarose beads. This antibody is specific for mouse Stat5 protein. A BLAST analysis was used to suggest cross-reactivity with Stat5 from human, mouse and rat based on 100% homology with the immunizing sequence. Cross-reactivity with Stat5 from other sources has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 91 kDa in size corresponding to phosphorylated Stat5 protein by western blotting in the appropriate cell lysate or extract.
Tested Applications
ELISA, WB
NCBI Accession Number
6755672
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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