SMAD4 Antibody

Rabbit anti-SMAD4 antibody, SMAD-4, SMAD 4, mothers against decapentaplegic homolog 4 antibody, MAD homolog 4, Mothers against DPP homolog 4, Deletion target in pancreatic carcinoma 4 antibody, SMAD family member 4, DPC 4, DPC4, MADH4, MADH 4

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human SMAD4 protein.

This affinity purified antibody is directed against human SMAD4 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with SMAD4 protein from human, mouse and Xenopus based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 60 kDa in size corresponding to SMAD4 protein by western blotting in the appropriate cell lysate or extract.