RSL1D1 Antibody
PBK1 antibody
Product Specifications
Product Name Alternative
Rabbit anti-PBK1 antibody, PBK-1, PBK 1, CATX-11, CATX11 antibody, RSL1D1, Cellular senescence inhibited gene protein antibody, CSIG antibody, L12 antibody, Ribosomal L1 domain containing protein 1 antibody
UniProt
O76021
Reactivity
Human
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of human PBK1.
Target
RSL1D1
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1.1 mg/mL
Dilution
ELISA: 1:2,500 - 1:10,000, IF: 1:100, IP: 1:100, WB: 1:500 - 1:2,000
Purity
This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for human PBK1 protein. A BLAST analysis was used to suggest limited cross-reactivity with PBK1 from mouse based on a 75% homology with the immunizing sequence. Cross-reactivity with PBK1 from other sources has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This affinity purified antibody has been tested for use in ELISA, immunoprecipitation, immunofluorescence microscopy and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 55 kDa in size corresponding to PBK1 by western blotting in the appropriate cell lysate or extract. This antibody is capable of detecting both over-expressed and endogenous PBK1. For immunofluorescence microscopy, fix cells with methanol.
Tested Applications
ELISA, IF, IP, WB
NCBI Accession Number
62901068
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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