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UBE2J1 Antibody

UBE2J1 antibody

Product Specifications

Product Name Alternative

Rabbit anti-UBE2J1 antibody, rabbit anti-E2 J1 antibody, rabbit anti-Ubiquitin conjugating enzyme E2J1 antibody, Non-canonical ubiquitin-conjugating enzyme 1, Ube2j1, E2 ubiquitin-conjugating enzyme J1, NCUBE-1, Yeast ubiquitin-conjugating enzyme UBC6 homolog E, HsUBC6e

UniProt

Q9Y385

Reactivity

Human

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues near the amino terminus of the human Ube2j1 protein.

Target

UBE2J1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cell Biology

Concentration

0.71 mg/mL

Dilution

ELISA: 1:1,000 - 1:5,000, IP: 1-2 μg, WB: 1:200 - 1:2,000

Purity

This affinity purified antibody is directed against human Ube2j1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with Ube2j1 protein from human, mouse and rat based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This affinity purified antibody has been tested for use in ELISA, western blotting and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 35-40 kDa in size corresponding to Ube2j1 protein by western blotting in the appropriate cell lysate or extract.

Tested Applications

ELISA, IP, WB

NCBI Accession Number

37577122

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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