Mdm2 Antibody

Rabbit anti-MDM2 antibody, MDM-2, E3 ubiquitin-protein ligase Mdm2, p53-binding protein Mdm2, Oncoprotein Mdm2, Double minute 2 protein, RING-type E3 ubiquitin transferase Mdm2, Double minute 2 protein, Hdm2

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 175-200 of mouse MDM2.

This affinity-purified antibody is directed against mouse MDM2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Reactivity occurs against Mouse MDM2 protein. A BLAST analysis was used to suggest minimal cross reactivity with MDM2 homologues from other sources.

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 90 kDa in size corresponding to MDM2 protein by western blotting in the appropriate cell lysate or extract. See Saucedo, et al (1999) for a discussion on expected molecular weights.