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MER2, REC107 Antibody

Mer2 antibody

Product Specifications

Product Name Alternative

Rabbit anti-Mer2 antibody, Meiotic recombination 2 protein, Recombination protein 107, REC107, YJR021C, J1462

UniProt

P21651

Reactivity

Yeast

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 26-35 of Saccharomyces cerevisiae Mer2 protein.

Target

MER2, REC107

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Signal Transduction

Concentration

0.75 mg/mL

Dilution

ELISA: 1:5,000 - 1:25,000, IP: 1:100, WB: 1:1,000 - 1:10,000

Purity

This affinity-purified antibody is directed against the Saccharomyces cerevisiae Mer2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Reactivity occurs against Saccharomyces cerevisiae Mer2 protein and reactivity is independent of phosphorylation at residue S30. A BLAST analysis was used to suggest minimal cross reactivity with Mer2 homologues from other sources.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 50 kDa in size corresponding to Mer2 protein by western blotting in the appropriate cell lysate or extract. This antibody is reactive with both phosphorylated and unphosphorylated Mer2 at the S30 position.

Tested Applications

ELISA, IP, WB

NCBI Accession Number

1170924

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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