Gli2 Antibody
Gli-2 antibody
Product Specifications
Product Name Alternative
Rabbit anti-Gli-2 antibody, Gli 2, Gli2, zinc finger protein GLI2, Tax helper protein antibody, Thp antibody
UniProt
Q8K0K3
Reactivity
Mouse
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids from an internal region of Mouse Gli-2.
Target
Gli2
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Stem Cell & Developmental Biology
Concentration
1.02 mg/mL
Dilution
ELISA: 1:15,000 - 1:60,000, IHC: 2 μg/ml to 20 μg/ml, WB: 1:500 - 1:2,000
Purity
This affinity purified antibody is directed against mouse Gli-2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with Gli-2 from mouse and rat sources based on 100% sequence homology with the immunogen. Reactivity with Gli-2 from other sources is not known.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. See figure legend for expectations by WB and IHC. Multiple splice variants have been reported for this protein.
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
124487481
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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