EGR1 Antibody
EGR-1 antibody
Product Specifications
Product Name Alternative
Rabbit anti-EGR-1 Antibody, EGR1, EGR 1, AT225 antibody, Early growth response 1 antibody, KROX24 antibody, Nerve growth factor-induced protein A antibody, NGFI-A, Transcription factor ETR103, Transcription factor Zif268, ZNF225, Zinc finger protein 225, Zinc finger protein Krox-24
UniProt
P18146
Reactivity
Human, Mouse
Immunogen
This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 85-115 of Human EGR-1.
Target
EGR1
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
0.93 mg/mL
Dilution
ELISA: 1:4,000 - 1:16,000, IHC: 2 μg/ml to 20 μg/ml, WB: 1:500 - 1:3,000
Purity
This affinity purified antibody is directed against human EGR-1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee sources based on 100% homology for the immunogen sequence. This antibody is expected to cross react with EGR-1.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~58 kDa in size corresponding to EGR-1 by western blotting in the appropriate cell lysate or extract.
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_001955.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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