PARK7 Antibody
PARK-7 antibody
Product Specifications
Product Name Alternative
Rabbit anti-PARK7 antibody, PARK-7, PARK 7, Protein DJ-1, Parkinson disease protein 7, Parkinsonism-associated deglycase, DJ-1 mutant, oncogene DJ1, DJ-1 oncogene product
UniProt
Q99497
Reactivity
Human
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminus of Human PARK7 protein.
Target
PARK7
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Concentration
1.3 mg/mL
Dilution
ELISA: 1:10,000 - 1:50,000, IHC: 2 mg/ml - 5 μg/ml, WB: 1:500 - 1:2,000
Purity
This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human PARK7 protein. BLAST analysis was used to determine that 100% homology with the immunizing peptide sequence is on record for this protein from human, chimpanzee, African green monkey, zebrafish and also for mutant/variant forms of human DJ-1 protein. Cross reactivity with PARK7 from frog, mouse, rat, dog, chicken, Japanese rice fish and Atlantic salmon may also occur as the sequence varies by only one amino acid residue as indicated by BLAST analysis. Reactivity with PARK7 protein from other sources is not known.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 28 kDa in size corresponding to PARK7 by western blotting in the appropriate cell lysate or extract.
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
31543380
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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