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PARK7 Antibody

PARK-7 antibody

Product Specifications

Product Name Alternative

Rabbit anti-PARK7 antibody, PARK-7, PARK 7, Protein DJ-1, Parkinson disease protein 7, Parkinsonism-associated deglycase, DJ-1 mutant, oncogene DJ1, DJ-1 oncogene product

UniProt

Q99497

Reactivity

Human

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminus of Human PARK7 protein.

Target

PARK7

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cell Biology

Concentration

1.3 mg/mL

Dilution

ELISA: 1:10,000 - 1:50,000, IHC: 2 mg/ml - 5 μg/ml, WB: 1:500 - 1:2,000

Purity

This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human PARK7 protein. BLAST analysis was used to determine that 100% homology with the immunizing peptide sequence is on record for this protein from human, chimpanzee, African green monkey, zebrafish and also for mutant/variant forms of human DJ-1 protein. Cross reactivity with PARK7 from frog, mouse, rat, dog, chicken, Japanese rice fish and Atlantic salmon may also occur as the sequence varies by only one amino acid residue as indicated by BLAST analysis. Reactivity with PARK7 protein from other sources is not known.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 28 kDa in size corresponding to PARK7 by western blotting in the appropriate cell lysate or extract.

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

31543380

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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