SETDB1 Antibody
ESET antibody
Product Specifications
Product Name Alternative
Rabbit anti-ESET antibody, ERG-associated protein with SET domain, rabbit anti-SETDB1 antibody, SETDB-1, SETDB 1, Histone lysine N methyltransferase antibody, Histone-lysine N-methyltransferase SETDB1 antibody, KMT1E antibody, Lysine N-methyltransferase 1E antibody, SET domain bifurcated 1, H3-K9-HMTase 4, Histone H3-K9 methyltransferase 4
UniProt
Q15047
Reactivity
Human
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1050-1075 of human ESET.
Target
SETDB1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Epigenetics & Chromatin
Concentration
1.17 mg/mL
Dilution
ELISA: 1:10,000 - 1:50,000, WB: 1:1,000 - 1:2,500
Purity
This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human ESET protein.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This affinity purified antibody has been tested for use in ELISA and western blotting. Western blotting shows reactivity specific for human ESET detecting a band at approximately 170 kDa. Specific band detection by western blot is blocked by peptide competition by pre-incubating the antibody with the immunizing peptide prior to reaction with the membrane. Reactivity in other immunoassays is unknown.
Tested Applications
ELISA, WB
NCBI Accession Number
224177469
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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