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SETDB1 Antibody

ESET antibody

Product Specifications

Product Name Alternative

Rabbit anti-ESET antibody, ERG-associated protein with SET domain, rabbit anti-SETDB1 antibody, SETDB-1, SETDB 1, Histone lysine N methyltransferase antibody, Histone-lysine N-methyltransferase SETDB1 antibody, KMT1E antibody, Lysine N-methyltransferase 1E antibody, SET domain bifurcated 1, H3-K9-HMTase 4, Histone H3-K9 methyltransferase 4

UniProt

Q15047

Reactivity

Human

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1050-1075 of human ESET.

Target

SETDB1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Epigenetics & Chromatin

Concentration

1.17 mg/mL

Dilution

ELISA: 1:10,000 - 1:50,000, WB: 1:1,000 - 1:2,500

Purity

This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human ESET protein.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This affinity purified antibody has been tested for use in ELISA and western blotting. Western blotting shows reactivity specific for human ESET detecting a band at approximately 170 kDa. Specific band detection by western blot is blocked by peptide competition by pre-incubating the antibody with the immunizing peptide prior to reaction with the membrane. Reactivity in other immunoassays is unknown.

Tested Applications

ELISA, WB

NCBI Accession Number

224177469

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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