NCAPH Antibody

Rabbit anti-BRRN-1 Antibody, BRRN1, BRRN 1, Barren Drosophila Homolog of 1 antibody, Barren homolog protein 1 antibody, BRRN antibody, CAP H antibody, CAPH antibody, Chromosome associated protein H antibody, Condensin complex subunit 2 antibody, Condensin subunit 2 antibody

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 435-460 of Human BRRN-1.

This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human BRRN1 protein. However, BLAST analysis indicates 100% homology (18/18) for this protein from human, and chimpanzee. Partial cross reactivity is expected against mouse, dog, rat chicken, and from based on sequence homology of the amino terminal 13 amino acids of the immunogen sequence. The core 8 amino acids of the immunogen, A-G-P-D-H-W-R-F, show 100% identity with numerous proteins from a variety of organisms. If blots show multiple banding patterns in any particular lysate, it is suggested to confirm the specific reactivity against the BRRN1 protein using peptide competition. Reactivity with Brnn1 proteins from other sources is not known.

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 83 kDa in size corresponding to human BRRN1 by western blotting in the appropriate cell lysate or extract.