RAD52 Antibody

Rabbit anti-RAD52 antibody, RAD-52, RAD 52, DNA repair protein RAD52 homolog antibody, RAD 52 antibody, RAD52 homolog antibody, recombination protein RAD52 antibody

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 350-375 of the Human Rad 52 protein.

This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human RAD52 protein.

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity purified antibody has been tested by WB and ELISA. Anti-RAD52 is useful in western blotting using HeLa nuclear extracts. Dilutions for western blotting represent a starting point dilution and further optimization may be required. The antibody detects a band of approximately 63.0 kDa (predicted molecular weight: 46.3 kDa) . Specific band detection by western blot is blocked by peptide competition by pre-incubating the antibody with the immunizing peptide prior to reaction with the membrane. Reactivity in other immunoassays is unknown.