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RAD9 Antibody

Rad9 S1260 antibody

Product Specifications

Product Name Alternative

Rabbit anti-Rad9 antibody, Rabbit anti-Rad9 S1260 antibody, Rad 9, Rad-9, Cell cycle checkpoint control protein antibody, Cell cycle checkpoint control protein RAD9A antibody, DNA repair exonuclease rad9 homolog A antibody, DNA repair protein RAD9, YDR217C, YD9934.02C

UniProt

P14737

Reactivity

Yeast

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to phosphorylated form of an internal region near aa 1225-1275 from the aa1309 yeast Rad9 protein conjugated to KLH.

Target

RAD9

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

1.1 mg/mL

Dilution

ELISA: 1:10,000 - 1:50,000, WB: 1:500- 1:2,000

Purity

This is an affinity purified antibody directed against yeast Rad9 produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. No reactivity is expected against the human analog of RAD9. Reactivity against RAD9 from other sources is unknown.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This affinity purified antibody has been tested for use in ELISA against the immunizing peptide. Reactivity in other immunoassays is unknown. Mature RAD9 is reported to have an apparent molecular weight of ~140kDa.

Tested Applications

ELISA, WB

NCBI Accession Number

NP_010503.1

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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