DNMT3L Antibody
DNMT3L antibody
Product Specifications
Product Name Alternative
Rabbit anti-DNMT3L antibody, DNA (cytosine-5) -methyltransferase 3-like, DNMT3L, DNMT 3L, DNMT-3L
UniProt
Q9UJW3
Reactivity
Human
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 150-175 of Human DNMT3L (DNA (cytosine-5-) -methyltransferase 3-like) . DNMT3L is a nuclear protein with similarity to DNA methyltransferases.
Target
DNMT3L
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Stem Cell & Developmental Biology
Concentration
1.11 mg/mL
Dilution
ELISA: 1:1000 - 1:3000, IHC: 1:500, IP: 1:100, WB: 1:500
Purity
This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. This antibody is expected to cross-react with mouse as the sequence of the human immunogen shows 76% identity with mouse. Reactivity with DNMT3L from other species has not yet been tested.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-DNMT3L antibody has been tested for use in ELISA against the immunizing peptide. This antibody has been tested in immunohistochemistry and western blot. This antibody works for western blotting only following immunoprecipitation. The expected molecular weight of human DNMT3L is 43.6 kDa. Reactivity in other immunoassays is unknown.
Tested Applications
ELISA, IHC, IP, WB
NCBI Accession Number
NP_787063.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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