NEK2 Antibody

Rabbit anti-NEK2 antibody, Serine threonine protein kinase Nek2 antibody, HSPK 21 antibody, NEK 2 antibody, NEK2A antibody, NIMA, never in mitosis a-related kinase 2 antibody, NimA-like protein kinase 1 antibody, NIMA related kinase 2 antibody, NEK-2, NLK1, NLK-1, NEK2A, HSPK21

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 275-300 of Human NEK2 protein.

This product is an affinity-purified antibody produced by immunoaffinity chromatography using peptide coupled to agarose beads. BLAST analysis indicates 100 % homology of the immunizing sequence with NEK2 homologues from human and chimpanzee. Cross reactivity with NEK2 protein homologues from other sources may not occur as sequence homology varies by at least one amino acid residue in this sequence.

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 52 kDa in size corresponding to NEK2 protein by western blotting in the appropriate cell lysate or extract. Splice variants exist for this protein that may result in the detection of lower molecular weight bands.