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GSK3A Antibody

GSK3 Alpha antibody

Product Specifications

Product Name Alternative

Rabbit anti-GSK3 alpha antibody, rabbit anti-GSK3α antibody, GSK 3 alpha antibody, Glycogen synthase kinase-3 alpha antibody, GSK-3 alpha antibody, GSK 3A antibody, GSK-3A antibody, GSK3alpha antibody, Serine/threonine-protein kinase GSK3A

UniProt

P49840

Reactivity

Human

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N-Terminal region near aa 1-25 of human GSK3 alpha.

Target

GSK3A

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Signal Transduction

Concentration

1.0 mg/mL

Dilution

ELISA: 1:4,000 - 1:20,000, WB: 1:500 - 1:2,000

Purity

This affinity-purified antibody is directed against human GSK3A. The product was affinity purified from monospecific antiserum by immunoaffinity purification. This antibody will react with both the phosphorylated and non-phosphorylated form of the protein at the S21residue. A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee and rat based on 100% homology for the immunogen sequence. Cross reactivity with GSK3A homologues from other sources has not been determined. No cross reactivity is expected against GSK3B.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This affinity-purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 52 kDa in size corresponding to GSK3A by western blotting in the appropriate cell lysate or extract.

Tested Applications

ELISA, WB

NCBI Accession Number

49574532

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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