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PPARG Antibody

PPAR Gamma 1 + 2 antibody

Product Specifications

Product Name Alternative

Rabbit anti-PPAR gamma 1 and 2 antibody, Peroxisome proliferator-activated receptor gamma, Nuclear receptor subfamily 1 group C member 3, NR1C3, PPARG, PPAR gamma, PPARG1 antibody, PPARG2 antibody

UniProt

P37231

Reactivity

Canine, Duck, Guinea pig, Hamster, Human, Mink, Monkey, Mouse, Porcine, Primate, Rabbit, Rat, Rodent

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 250-300 of human PPAR gamma isoform 1.

Target

PPARG

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

1.1 mg/mL

Dilution

ELISA: 1:8,000 - 1:32,000

Purity

This affinity purified antibody is directed against human PPAR gamma protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest broad reactivity with this protein from several sources. Expect reactivity against human (g1 and g2), mouse (g1 and g2), rat, squirrel, orangutan, duck (g1), macaque (g1-g7), boar (g1a-1d), mink, guinea pig, rabbit (g1 and g3), dog and hamster sources based on 100% homology for the immunogen sequence. Cross reactivity with PPAR gamma homologues from other sources has not been determined. No reactivity is expected against other subtypes of PPAR.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This affinity-purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user.

Tested Applications

ELISA, WB

NCBI Accession Number

20336229

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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