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Esrp1 Antibody

Esrp-1 antibody

Product Specifications

Product Name Alternative

Mouse anti-Esrp-1 antibody, mouse anti-Esrp1 antibody, Rbm35a, Epithelial splicing regulatory protein 1, RNA-binding protein 35A, RNA-binding motif protein 35A

UniProt

Q3US41

Reactivity

Mouse

Immunogen

Anti-Esrp-1 was produced by repeated immunizations of full length recombinant mouse Esrp-1 fusion protein.

Target

Esrp1

Clonality

Monoclonal

Clone

27H12.F3.H5

Conjugation

Unconjugated

Concentration

1.0 mg/mL

Dilution

ELISA: 1:750,000, WB: 1:1000

Purity

This product is an IgG fraction antibody purified by Protein-A chromatography followed by extensive dialysis against the buffer stated above. This antibody reacts with mouse Esrp-1 protein. A BLAST analysis of the immunizing protein sequence shows 100% homology with Esrp-1 from mouse and a 91% sequence homology with Esrp-1 from human, pig, rat, opossum, horse, cattle, panda, dog, and chimpanzee. The binding epitope of this monoclonal antibody has not been mapped.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This protein-A purified antibody has been tested for use western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 75.5 kDa in size corresponding to Esrp-1 by western blotting in the appropriate cell lysate or extract.

Tested Applications

ELISA, WB

NCBI Accession Number

NP_918944.2

Host or Source

Mouse

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG2b

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