GDF15 Antibody

Rat anti-NAG1 antibody, NAG-1, GDF15, MIC-1, nonsteroidal anti-inflammatory drug-activated gene, NSAID-activated gene 1 protein, growth differentiation factor 15, macrophage inhibitory compound 1, prostate-derived factor

This Protein-A purified antibody was prepared by repeated immunizations with an MBP-tagged recombinant protein produced in E.coli corresponding to C-term mouse NAG-1 protein. Cross reactivity to MBP is negative.

This product was purified from concentrated tissue culture supernatant Protein G chromatography. This antibody reacts with the C-terminus of endogenous NAG-1 protein from mouse tissues. A BLAST analysis suggests reactivity with NAG-1 from chimpanzee and macaque based on a 100% homology. Partial reactivity is expected against rat based on an 86% homology with the immunizing sequence. Cross-reactivity with NAG-1 from other sources has not been determined.

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

This Protein G Anti-NAG1 purified antibody has been tested by ELISA, SDS-PAGE, and western blot for mouse NAG-1 protein. Specific conditions for reactivity should be optimized by the end user. Expect bands in Western blots of native protein of approximately ~58kDa using the appropriate cell lysate or extract.