Ahsa1 Antibody
AHA1 antibody
Product Specifications
Product Name Alternative
Rat anti-Aha1 Antibody, Aha1, Ahsa1 antibody, Activator of Hsp90 ATPase, Activator of 90 kDa heat shock protein ATPase homolog 1 antibody
UniProt
Q8BK64
Reactivity
Human
Immunogen
This Protein G purified monoclonal antibody was produced in rats by repeated immunizations with full length recombinant mouse AHA1 protein followed by hybridoma development.
Target
Ahsa1
Clonality
Monoclonal
Clone
25F2.D9
Conjugation
Unconjugated
Field of Research
Cell Biology
Concentration
1.0 mg/mL
Dilution
ELISA: 1:20,000, IHC: 5-10 μg/mL, WB: 1:1,000
Purity
This product is an IgG fraction antibody purified from tissue culture supernatant by Protein-G chromatography, followed by extensive dialysis against the buffer stated above. It is directed against human Aha1 protein. A BLAST analysis was used to suggest cross-reactivity with Aha1 protein from mouse, human and chimpanzee based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This Protein-G purified antibody has been tested for use in ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 38-40 kDa in size corresponding to Aha1 protein by western blotting in the appropriate cell lysate or extract.
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_666148.1
Host or Source
Rat
Preservative
Preservative: None. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG2a
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